Complement Resistance, as Determined by Viable Count and Flow Cytometric Methods, and Its Association with the Presence of iss and the Virulence of Avian Escherichia coli

2002 ◽  
Vol 46 (2) ◽  
pp. 386-392 ◽  
Author(s):  
Lisa K. Nolan ◽  
Catherine W. Giddings ◽  
Shelley M. Horne ◽  
Curt Doetkott ◽  
Penelope S. Gibbs ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Viable Count ◽  
Complement Resistance ◽  
Flow Cytometric

Contribution of the outer membrane protein OmpW in Escherichia coli to complement resistance from binding to factor H

2016 ◽  
Vol 98 ◽  
pp. 57-62 ◽  
Author(s):  
Weiyan Li ◽  
Liangyou Wen ◽  
Chuchu Li ◽  
Ran Chen ◽  
Zhicang Ye ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Factor H ◽  
Complement Resistance

scholarly journals Detection and identification of public health important pathogens present in fruit salads sold on Lagos State University campus

2020 ◽  
pp. 11-16
Author(s):  
Adedayo Emmanuel Ogunware ◽  
Odubiyi Opeyemi Esther ◽  
Pelumi Abimbola Adedigba ◽  
Adesewa Adeyinka Adekoya
Keyword(s):  
Escherichia Coli ◽  
Total Coliform ◽  
Viable Count ◽  
State University ◽  
University Campus ◽  
Penicillium Species ◽  
Culture Techniques ◽  
Detection And Identification ◽  
Lagos State ◽  
Bacillus Spp

A total of fifteen pre-packaged fruit salad samples containing pineapple, water melon, pawpaw and cucumber sold in the Lagos State University, Ojo Campus was analyzed using culture techniques for its microbial qualities. Five bacteria genera isolates obtained are Bacillus spp, Staphylococcus aureus, Pseudomonas spp, Escherichia coli, Streptococcus and the three fungi genera isolates are Aspergillus species, Penicillium species, and Saccharomyces cerevisiae. Escherichia coli had the highest frequency of (40%) followed by Streptococcus with (20%), Staphylococcus, Bacillus, Pseudomonas has the same frequency of (13%). The total viable count was in the range of 1.6 × 105 cfu/g to 5.65 × 105 cfu/g while the total coliform count ranged from 1.0 × 105 to 3.3 × 105 cfu/g. The fungal count ranged from 1.5 × 105 to 3.4 × 105 cfu/g. This study revealed that fruit salads in the studied area needs proper sanitation practice during processing in order to avoid risks associated with the consumption of contaminated fruits for the consumers.

Survival of Bacteria in “Soul Foods” at 10-Centigrade

1981 ◽  
Vol 44 (4) ◽  
pp. 271-274
Author(s):  
ADELLE W. STEWART
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Typhimurium ◽  
Food Samples ◽  
Viable Count ◽  
Inoculum Size ◽  
Farm Families ◽  
Farm Family ◽  
Collard Greens ◽  
Naturally Occurring

The fate of naturally occurring and added bacterial pathogens was determined in “soul foods” purchased at local supermarkets and farm families while the foods were stored under conditions simulating those used for retail distribution, home storage, and preparation before use. Viable count determinations for 10 samples at the end of a 5-day period at 10 C showed considerable decreases in comparison to the inoculum size, indicating that growth was not promoted. Escherichia coli survived in all the food samples but the populations decreased by 1 to 9 log cycles/g of food. Salmonella typhimurium survived in 59% of the food samples. Except for farm family collard greens and sausage (encased), Staphylococcus aureus remained viable in all of the foods tested an d was the only survivor in cracklings (cooked) obtained from both sources. Clostridium perfringens was detected in farm family sweet peas and 23% of the pig offal samples.

High-Pressure Transient Sensitization of Escherichia coli to Lysozyme and Nisin by Disruption of Outer-Membrane Permeability

1996 ◽  
Vol 59 (4) ◽  
pp. 350-355 ◽  
Author(s):  
KRISTEL J. A. HAUBEN ◽  
ELKE Y. WUYTACK ◽  
CARINE C. F. SOONTJENS ◽  
CHRIS W. MICHIELS
Keyword(s):  
Escherichia Coli ◽  
High Pressure ◽  
Outer Membrane ◽  
High Pressures ◽  
Ethylenediaminetetraacetic Acid ◽  
Viable Count ◽  
Water Soluble ◽  
Pressure Treatment ◽  
E Coli ◽  
Glucose Agar

Escherichia coli MG1655 suspensions in 10 mM phosphate buffer (pH 7.0) were subjected to high pressures in the range of 180 to 320 MPa for 15 min. Cell death was evident at 220 MPa and increased exponentially with pressure. Surviving populations were sublethally injured, as demonstrated by their reduced ability to form colonies on violet red bile glucose agar, a selective growth medium containing crystal violet and bile salts. During exposure to high pressure (> 180 MPa), cells were sensitive to lysozyme, nisin, and ethylenediaminetetraacetic acid (EDTA), as was apparent from an increased lethality of pressure in the presence of these agents. Sublethal injury in the surviving population was lower in the presence of nisin and lysozyme, but higher in the presence of EDTA. Combinations of EDTA with nisin or lysozyme present during pressure treatment increased lethality in an additive manner. However, the addition of lysozyme, nisin and/or EDTA to pressurized cell suspensions immediately after pressure treatment did not cause any viable count reduction. Finally, we observed leakage of the periplasmic enzyme β-lactamase from an ampicillin-resistant recombinant E. coli MG1655 under high pressure. These results suggest that high pressure transiently disrupts the permeability of the E. coli outer membrane for water-soluble proteins.

scholarly journals Accurate flow cytometric monitoring of Escherichia coli subpopulations on solid food treated with high pressure carbon dioxide

2014 ◽  
Vol 117 (2) ◽  
pp. 440-450 ◽  
Author(s):  
S. Tamburini ◽  
P. Foladori ◽  
G. Ferrentino ◽  
S. Spilimbergo ◽  
O. Jousson
Keyword(s):  
Escherichia Coli ◽  
Carbon Dioxide ◽  
High Pressure ◽  
Solid Food ◽  
Flow Cytometric

scholarly journals The microbiology of cooked prawns and shrimps on retail sale

1985 ◽  
Vol 94 (3) ◽  
pp. 319-326 ◽  
Author(s):  
M. H. Greenwood ◽  
E. F. C. Coetzee ◽  
B. M. Ford ◽  
P. Gill ◽  
W. L. Hooper ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Vibrio Parahaemolyticus ◽  
Food Poisoning ◽  
Viable Count ◽  
Total Viable Count ◽  
Retail Sale ◽  
Point Of Sale ◽  
Three Samples ◽  
Laboratory Survey

SUMMARYIn an inter-laboratory survey, 148 samples of cooked prawns and shrimps were obtained at the point of sale to the consumer. Salmonellae andVibrio parahaemolyticuswere not detected.Yersinia enterocoliticawas isolated from three samples. Results for total viable count and presence ofEscherichia coliandStaphylococcus aureuscomplied well with available guidelines for imported cooked prawns, suggesting that the risk of food poisoning from retail samples of these foods in the South of England is minimal.

scholarly journals Transformation of Rickettsia prowazekii to Erythromycin Resistance Encoded by the Escherichia coli ereB Gene

2000 ◽  
Vol 182 (11) ◽  
pp. 3289-3291 ◽  
Author(s):  
Lyudmila I. Rachek ◽  
Andria Hines ◽  
Aimee M. Tucker ◽  
Herbert H. Winkler ◽  
David O. Wood
Keyword(s):  
Escherichia Coli ◽  
Fluorescent Protein ◽  
Flow Cytometric Analysis ◽  
Etiologic Agent ◽  
Erythromycin Resistance ◽  
Rickettsia Prowazekii ◽  
Flow Cytometric ◽  
Epidemic Typhus ◽  
Green Fluorescent

ABSTRACT Rickettsia prowazekii, the etiologic agent of epidemic typhus, is an obligate, intracytoplasmic, parasitic bacterium. Recently, the transformation of this bacterium via electroporation has been reported. However, in these studies identification of transformants was dependent upon either selection of an R. prowazekii rpoB chromosomal mutation imparting rifampin resistance or expression of the green fluorescent protein and flow cytometric analysis. In this paper we describe the expression inR. prowazekii of the Escherichia coli ereBgene. This gene codes for an erythromycin esterase that cleaves erythromycin. To the best of our knowledge, this is the first report of the expression of a nonrickettsial, antibiotic-selectable gene inR. prowazekii. The availability of a positive selection for rickettsial transformants is an important step in the characterization of genetic analysis systems in the rickettsiae.

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